Determination of antibiotic resistance and biofilm formation in Klebsiella strains isolated from bovine mastitis cases

Mastitis is diseases of dairy cows with a high economic impact. Bovine mastitis caused by wide range bacterial pathogens. As one the major environmental pathogens Klebsiella ssp. was investigated in this study some phenotypic characteristics like antibiotic resistance patterns and biofilm formation properties. A number 483 farms around Konya were examined California Test (CMT) producing 36 positive terms subclinical mastitis. further 19 samples from clinical mastitic udders also collected. Samples inoculated onto Trypticase Soy Agar medium enriched sheep blood incubated aerobicaly for 24-48 hours at 37 °C. By morphological, biochemical cultural charcateristics 14 isolates out coliform identified as The double disc synergy method Congo Red test used to perform susceptibility vitro slime forming properties, respectively. Resistances antibiotics Ampicillin, Carbenicillin, Cephotaxime, Chloramphenicol, Erythromycin, Gentamicin, Neomycin, Oxytetracycline, Sulphamethoxazole/Trimpethoprim, Amoxicillin-Clavulanate Imipenem 78.5%, 35.7%, 42.8%, 100%, 7%, 50%, 14%, 21% Three total isolets produced only. This appears first report on ESBL ssp cases bovine Konya, Türkiye. Presently, two numbers antimicrobial combinations treat are recommended work. In conclusion, because costly challange nature implemantation an effective control program should be local Konya.